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Agro-Science
Journal of Tropical Agriculture, Food, Environment and Extension
 

ISSN 1119-7455
   
 
         
 
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Article No 1 of Volume 3.1 (2004)
 
    
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METABOLIC FINGER PRINTS OF SOIL BACTERIA; INFLUENCE OF ORGANIC AND INORGANIC AMENDMENTS

R.C. Eneje1*, J.S.C, Mbagwu 2 and H Insam3

1Department of Soil Science and Agroclimatology

Michael Okpara University of Agriculture Umudike,  Nigeria.

 

2Department of Soil Science

University of Nigeria, Nsukka, Nigeria.

 

3 Institute of Microbiology

University of Innsbruck, Austria.

 

Abstract

The effect of organic and inorganic manure amendments on the metabolic finger prints of bacteria from soil samples taken from 0-2 cm depth of a humid tropical agricultural soil (University of Nigeria Nsukka research farm) was assessed. The manures (rice straw, (RW), poultry manure (PM) and fertilizer N. P.K. (12: 12: 17) were incorporated into the soil in the field and samples were collected after three, six, nine and twelve months for Biolog ecoplate analysis in the laboratory. Data of average well color density (AWCD) from the ecoplates twelve hourly readings were subjected to multivariate analysis of variance (MANOVA) after normalization with the Garland-mills scaling. Results indicated that the combined application of RW+ NPK increased the type and number of microorganisms in the soil by increasing the community of generalist for the carbon source utilization. The principal component analysis (PCA) of F1 and F2 using the standardized AWCD values indicated a shift from generalist to specialist C- source utilization with increased incubation period. There was a temporal and spatial difference in C availability and utilization pattern of C in the unamended or amended ecoplate samples.

 

Keywords: organic and inorganic manures, metabolic finger prints, AWCD, C- source utilization, PCA.

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Date:19/07/2018
 
     
 
 
 
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